Author:
Christine Pasero, Gwenaelle Gravis, Palma Rocchi, Sylvaine Just-Landi, Philippe Livrati, Jeanne Thomassin, Serge Brunelle, Naji Salem, Jochen Walz, Daniel Olive
Publication Date:
20 May 2012
Source:
Journal of Clinical Oncology
Description:
e15155 Background: Recently, therapeutic vaccines and monoclonal antibody-based therapies have been investigated as promising new treatments against prostate cancer (PC). Natural killer (NK) cells which have anti-tumoral activity thus represent promising candidates for immunotherapy. Our group have reported major impairments of NK in acute myeloid leukemia and recently in metastatic breast cancer. The aim of this work was to evaluate the phenotype and function of NK cells in patients with localized and metastatic PC to determine their role in disease progression. Methods: Activating and inhibitory receptors were analyzed in peripheral NK cells from 14 patients with localized PC, 33 with metastatic disease and 30 healthy donors. NK function was evaluated by measuring CD107 degranulation. Results: The ratio mean fluorescence intensity RMFI (MFI receptor/MFI control isotype) of the activating receptor …
Author:
Xiaoxuan Liu, Cheng Liu, Erik Laurini, Paola Posocco, Sabrina Pricl, Fanqi Qu, Palma Rocchi, Ling Peng
Publication Date:
05 Mar 2012
Source:
Molecular Pharmaceutics
Description:
Successful achievement of RNA interference in therapeutic applications requires safe and efficient vectors for siRNA delivery. In the present study, we demonstrate that a triethanolamine (TEA)-core PAMAM dendrimer of generation 5 (G5) is able to deliver sticky siRNAs bearing complementary An/Tn 3′-overhangs effectively to a prostate cancer model in vitro and in vivo and produce potent gene silencing of the heat shock protein 27, leading to a notable anticancer effect. The complementary An/Tn (n = 5 or 7) overhangs characteristic of these sticky siRNA molecules help the siRNA molecules self-assemble into “gene-like” longer double-stranded RNAs thus endowing a low generation dendrimer such as G5 with greater delivery capacity. In addition, the An/Tn (n = 5 or 7) overhangs act as protruding molecular arms that allow the siRNA molecule to enwrap the dendrimer and promote a better interaction and …
Author:
Yuting Fan, Yi Xia, Jingjie Tang, Fabio Ziarelli, Fanqi Qu, Palma Rocchi, Juan L Iovanna, Ling Peng
Publication Date:
20 Feb 2012
Source:
Chemistry–A European Journal
Description:
A mixed-ligand system of Pd/Synphos/Xantphos promotes effective C N coupling in the synthesis of various N-arylaminotriazole and N-arylaminopurine nucleoside analogues. This catalytic system is strikingly powerful and efficient, allowing for unparalleled substrate scope and high product yields as well as promotion of C Cl bond activation for C N coupling. 31 P NMR investigations provided useful evidence favoring a mechanism in which the catalytic reactions are mediated by ligand exchange during both Pd catalyst formation and oxidative insertion. For more details see the Communication by L. Peng et al. on page 2221 ff.
Author:
Sophie Giusiano, Stéphane Garcia, Claudia Andrieu, Nelson Javier Dusetti, Cyrille Bastide, Martin Gleave, Colette Taranger‐Charpin, Juan Lucio Iovanna, Palma Rocchi
Publication Date:
01 Feb 2012
Description:
BACKGROUND Tumor protein 53‐induced nuclear protein 1 (TP53INP1) is a proapoptotic protein involved in cell stress response. Whereas there is an overexpression of TP53INP1 in numerous tissues submitted to stress agents, TP53INP1 is down‐expressed in stomach, pancreatic, and inflammation–mediated colic carcinomas. In medullary thyroid carcinomas, TP53INP1 overexpression correlates with poor prognosis. TP53INP1 expression has never been reported in Prostate Cancer (PC). Our aim was to investigate variations of TP53INP1 expression and their correlation to clinicopathological parameters in PC. METHODS Quantitative measurements of immunohistochemical expression of TP53INP1 using high‐throughput densitometry, assessed on digitized microscopic tissue micro‐array images, were correlated with clinicopathological parameters in 91 human PC. Treatment of LNCaP tumor cells in vitro …
Author:
Yuting Fan, Yi Xia, Jingjie Tang, Fabio Ziarelli, Fanqi Qu, Palma Rocchi, Juan L Iovanna, Ling Peng
Publication Date:
20 Jan 2012
Source:
Chemistry (Weinheim an der Bergstrasse, Germany)
Description:
Make it unique! A mixed-ligand system of Pd/Synphos/Xantphos promotes effective CN coupling in the synthesis of various N-arylaminotriazole and N-arylaminopurine nucleoside analogues. This catalytic system is strikingly powerful and efficient, allowing for unparalleled substrate scope and high product yields as well as promotion of C-Cl bond activation for CN coupling (see scheme).
Author:
Xiaoxuan Liu, Palma Rocchi, Ling Peng
Publication Date:
01 Jan 2012
Source:
New Journal of Chemistry
Description:
There is a tremendous interest in moving siRNA therapeutics into a clinical setting for the treatment of various diseases. This in itself however depends largely on the availability of safe and efficient siRNA delivery systems. In this context, dendrimers have attracted considerable attention as siRNA vectors due to their well-defined structures and multivalent features. The present review offers a brief overview of the current status of dendrimers as siRNA delivery vectors, focusing on the different dendrimers investigated for their siRNA delivery ability and the related structural alterations employed to improve their safety and efficiency for this purpose.
Author:
Yi Xia, Palma Rocchi, Juan L Iovanna, Ling Peng
Publication Date:
01 Jan 2012
Source:
Drug discovery today
Description:
Pancreatic cancer belongs to the group of extremely aggressive human cancers; conventional cancer treatments have little impact. Increasing understanding of the pathways associated with pancreatic cancer progression has enabled the development of targeted therapy on this cancer. Heat shock proteins (HSPs) and related heat shock response (HSR) pathways control multiple important oncogenic pathways for pancreatic cancer development. Consequently, they represent promising novel targets for pancreatic cancer therapy. Various strategies have been proposed and elaborated to target HSPs/HSR in pancreatic cancer with the corresponding modulators, the details of which are highlighted in this review.
Author:
V Baylot, C Andrieu, M Katsogiannou, D Taieb, S Garcia, S Giusiano, J Acunzo, J Iovanna, M Gleave, C Garrido, P Rocchi
Publication Date:
01 Oct 2011
Source:
Cell death & disease
Description:
Despite many advances in oncology, almost all patients with pancreatic cancer (PC) die of the disease. Molecularly targeted agents are offering hope for their potential role in helping translate the improved activity of combination chemotherapy into improved survival. Heat shock protein 27 (Hsp27) is a chaperone implicated in several pathological processes such as cancer. Further, Hsp27 expression becomes highly upregulated in cancer cells after chemotherapy. Recently, a modified antisense oligonucleotide that is complementary to Hsp27 (OGX-427) has been developed, which inhibits Hsp27 expression and enhances drug efficacy in cancer xenograft models. Phase II clinical trials using OGX-427 in different cancers like breast, ovarian, bladder, prostate and lung are in progress in the United States and Canada. In this study, we demonstrate using TMA of 181 patients that Hsp27 expression and …
Author:
M Katsogiannou, Ling Peng, C V Catapano, P Rocchi
Publication Date:
01 Oct 2011
Source:
Current cancer drug targets
Description:
Castration-resistant prostate cancer remains incurable and a major cause of mortality worldwide. The absence of effective therapeutic approaches for advanced prostate cancer has led to an intensive search for novel treatments. Emerging nanomedical approaches have shown promising results, in vitro and in vivo, in improving drug distribution and bioavailability, tumor penetration and in limiting toxicity. Nanoscaled carriers bearing finely controlled size and surface properties such as liposomes, dendrimers and nanoparticles have been developed for successful passive and active tumortargeting. Enhanced pharmacokinetics of nanotherapeutics, through improved target delivery and prolonged tissue halflife provides optimal drug delivery that is tumor-specific. Tumor-targeting may be improved through ligand directed delivery systems binding to tumor-specific surface receptors improving cellular uptake through …
Author:
Xiaoxuan Liu, Miriam Yammine, Palma Rocchi, Catherine Nguyen, Ling Peng
Publication Date:
01 Jul 2011
