Author:
Claudia Andrieu, Gilles Karsenty, Claire Seillan, Palma Rocchi
Publication Date:
01 Mar 2008
Source:
METABOLISMES HORMONES DIABETES ET NUTRITION
Author:
Claudia Andrieu, Gilles Karsenty, Claire Seillan, Palma Rocchi
Publication Date:
01 Jan 2008
Source:
Métabolismes, hormones, diabètes et nutrition
Description:
Profils moléculaires des cancers de la prostate : Apport de la génomique en pathologie
endocrinienne CNRS Inist Pascal-Francis CNRS Pascal and Francis Bibliographic
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Profils moléculaires des cancers de la prostate : Apport de la génomique en pathologie
endocrinienne Other title Molecular profiling of prostate cancer (en) Author ANDRIEU,
Claudia 1 ; KARSENTY, Gilles 1 ; SEILLAN, Claire 1 ; ROCCHI, Palma 1 [1] Inserm U624 'stress
cellulaire' Parc scientifique et technologique de Luminy, Marseille, France Source
Métabolismes, hormones, diabètes et nutrition. 2008, Vol 12, Num 2 ; 76-82 [5 p.] ; ref : 28 …
Author:
Meritxell Gironella, Mylène Seux, Min-Jue Xie, Carla Cano, Richard Tomasini, Julien Gommeaux, Stephane Garcia, Jonathan Nowak, Man Lung Yeung, Kuan-Teh Jeang, Amandine Chaix, Ladan Fazli, Yoshiharu Motoo, Qing Wang, Palma Rocchi, Antonio Russo, Martin Gleave, Jean-Charles Dagorn, Juan L Iovanna, Alice Carrier, Marie-Josèphe Pébusque, Nelson J Dusetti
Publication Date:
09 Oct 2007
Source:
Proceedings of the National Academy of Sciences
Description:
Pancreatic cancer is a disease with an extremely poor prognosis. Tumor protein 53-induced nuclear protein 1 (TP53INP1) is a proapoptotic stress-induced p53 target gene. In this article, we show by immunohistochemical analysis that TP53INP1 expression is dramatically reduced in pancreatic ductal adenocarcinoma (PDAC) and this decrease occurs early during pancreatic cancer development. TP53INP1 reexpression in the pancreatic cancer-derived cell line MiaPaCa2 strongly reduced its capacity to form s.c., i.p., and intrapancreatic tumors in nude mice. This anti-tumoral capacity is, at least in part, due to the induction of caspase 3-mediated apoptosis. In addition, TP53INP1−/− mouse embryonic fibroblasts (MEFs) transformed with a retrovirus expressing E1A/rasV12 oncoproteins developed bigger tumors than TP53INP1+/+ transformed MEFs or TP53INP1−/− transformed MEFs with restored TP53INP1 …
Author:
Tanguy Seiwert, Palma Rocchi, Lynn Bracht, Varalakshmi Janamanchi, Ramasamy Jagadeeswaran, Aliya Husain, Dongfeng Tan, Li Qiang, Martin Gleave, Ravi Salgia
Publication Date:
01 Aug 2007
Source:
Journal of Thoracic Oncology
Description:
Purpose:To determine the role of HSP27 in non-small cell lung cancer (NSCLC) in tumor samples (expression) and in in-vitro and in-vivo models (overexpression/suppression).Methods:Tumor tissue microarrays (TMA) were stained for HSP27 and Phospho-HSP27 (IHC) and results correlated with outcome. Expression in metastases was compared with the respective matched primary tumors. HSP27 was overexpressed using lentivirus transfected A549 cells and compared with control sequence transfected A549. Cells were studied a) in-vivo for tumor growth, b) in-vitro for motility by time lapse microscopy. HSP27 was suppressed with specific siRNA (in-vitro) and HSP27 antisense (OGX-427; in-vitro and in-vivo). Normal human bronchial epithelial cells (NHBE) were used as controls (in-vitro). Cell cycle analysis was performed. Wortmannin and LY290042 were used to inhibit PI3K. Gene expression profile of HSP27 …
Author:
Tanguy Seiwert, Palma Rocchi, Lynn Bracht, Varalakshmi Janamanchi, Ramasamy Jagadeeswaran, Aliya Husain, Dongfeng Tan, Li Qiang, Martin Gleave, Ravi Salgia
Publication Date:
01 May 2007
Description:
1343 Purpose: To determine the role of HSP27 in non-small cell lung cancer (NSCLC) in tumor samples (expression), and in in-vitro and in-vivo models (overexpression/ suppression). Methods: Tumor tissue microarrays (TMA) were stained for HSP27 and Phospho-HSP27 (IHC) and results correlated with outcome. Expression in metastases was compared with the respective matched primary tumors. HSP27 was overexpressed using lentivirus transfected A549 cells and compared with control sequence transfected A549. Cells were studied a) in-vivo for tumor growth, b) in-vitro for motility by time lapse microscopy. HSP27 was suppressed with specific siRNA (in-vitro) and HSP27 antisense (OGX-427; in-vitro and in-vivo). Normal human bronchial epithelial cells (NHBE) were used as controls (in-vitro). Cell cycle analysis was performed. Wortmannin and LY290042 were used to inhibit PI3K. Results: HSP27 …
Author:
Masayuki Kamada, Alan So, Mototsugu Muramaki, Palma Rocchi, Eliana Beraldi, Martin Gleave
Publication Date:
01 Jan 2007
Source:
Molecular cancer therapeutics
Description:
Heat shock protein 27 (Hsp27) is a cytoprotective chaperone that is phosphoactivated during cell stress that prevents aggregation and/or regulate activity and degradation of certain client proteins. Recent evidence suggests that Hsp27 may be involved in tumor progression and the development of treatment resistance in various tumors, including bladder cancer. The purpose of this study was to examine, both in vitro and in vivo, the effects of overexpression of Hsp27 and, correspondingly, the down-regulation of Hsp27 using small interfering (si) RNA and OGX-427, a second-generation antisense oligonucleotide targeting Hsp27. Hsp27 overexpression increased UMUC-3 cell growth and resistance to paclitaxel. Both OGX-427 and Hsp27 siRNA decreased Hsp27 protein and mRNA levels by >90% in a dose- and sequence-specific manner in human bladder cancer UMUC-3 cells. OGX-427 or Hsp27 siRNA …
Author:
Palma Rocchi, Paul Jugpal, Alan So, Shannon Sinneman, Susan Ettinger, Ladan Fazli, Colleen Nelson, Martin Gleave
Publication Date:
01 Nov 2006
Source:
BJU international
Description:
OBJECTIVES To evaluate synthetic small interference RNA (siRNA) compounds targeting heat‐shock protein 27 (Hsp27) as an alternative approach to Hsp27 ‘knockdown’ in prostate cancer cells, as Hsp27 expression is highly up‐regulated in prostate cancer cells after androgen withdrawal or chemotherapy, to become uniformly highly expressed in androgen‐independent (AI) prostate cancer. MATERIALS AND METHODS We recently showed that targeting Hsp27 by a 2′‐methoxyethyl modified phosphorothioate antisense oligonucleotide, OGX‐427, inhibits Hsp27 expression and enhances hormone‐ and chemotherapy in prostate cancer xenograft models. In the present study, a ‘gene walk’ screening different siRNAs was initially used in PC‐3 and LNCaP cells to determine the most potent sequence to down‐regulate Hsp27 mRNA and protein levels. The effects of Hsp27 silencing on in vitro growth rates …
Author:
Kazuki Yamanaka, Palma Rocchi, Hideaki Miyake, Ladan Fazli, Alan So, UWE ZANGEMEISTER‐WITTKE, Martin E Gleave
Publication Date:
01 Jun 2006
Source:
BJU international
Description:
OBJECTIVE To determine whether a specifically designed bispecific (Bcl‐2/Bcl‐xL) antisense oligonucleotide (ASO) induces apoptosis and enhances chemosensitivity in human prostate cancer LNCaP cells, as Bcl‐2 and Bcl‐xL are both anti‐apoptotic genes associated with treatment resistance and tumour progression in many malignancies, including prostate cancer. MATERIALS AND METHODS Inhibition of Bcl‐2 and Bcl‐xL expression by the bispecific ASO was evaluated using real‐time reverse transcription‐polymerase chain reaction and Western blotting, while growth inhibition and induction of apoptosis were analysed by a crystal violet assay, flow cytometry and Western blotting of apoptosis‐relevant proteins. The effect of combined treatment with bispecific ASO and chemotherapy or small‐interference RNA (siRNA) targeting the clusterin gene was also investigated. RESULTS Bispecific ASO reduced …
Author:
Norihiro Hayashi, Susan Ettinger, Eliana Beraldi, Amina Zoubeidi, Ladan Fazli, Palma Rocchi, Colleen Nelson, Martin E Gleave
Publication Date:
01 Apr 2006
Source:
The Journal of Urology
Description:
RESULTS: Mouse weights were not significantly different between the 33% CR animals and the CMD mice. Seminal vesicle weights decrease in the CMD animals at 3 and 6 months compared to other groups and the ventral and dorsolateral prostates become hypoplastic. At the 6 month timepoint, 40% of the CMD animals developed liver tumors (excluded from the analysis). Global methylation loss (30%) was demonstrated in animals receiving the CMD versus CR diets. Expression analysis of IGF2 and H19 in the dorsolateral prostate demonstrates significant increases in expression at the 3 and 6 months compared to CR animals. No expression changes were noted for p57, another imprinted gene. Increased gene expression was mediated, in part, by an increase in the/GF2 P1, P2 and P3 promoters. Analyses demonstrate no significant changes in IGF2 imprinting in CMD animals compared to control …
Author:
Palma Rocchi, Eliana Beraldi, Susan Ettinger, Ladan Fazli, Robert L Vessella, Colleen Nelson, Martin Gleave
Publication Date:
01 Dec 2005
Description:
One strategy to improve therapies in prostate cancer involves targeting cytoprotective genes activated by androgen withdrawal to delay the emergence of the androgen-independent (AI) phenotype. The objectives of this study were to define changes in Hsp27 levels after androgen ablation and to evaluate the functional relevance of these changes in AI progression. Using a tissue microarray of 232 specimens of hormone-naïve and post-hormone ablation–treated prostate cancer, we found that Hsp27 levels increase after androgen ablation to become highly expressed (>4-fold, P ≤ 0.01) in AI tumors. Hsp27 overexpression rendered LNCaP cells highly resistant to androgen withdrawal both in vitro and in vivo. Tumor volume and serum prostate–specific antigen levels increased 4.3- and 10-fold faster after castration when Hsp27 was overexpressed. Treatment of LNCaP tumor cells in vitro with Hsp27 …
